Journal: JHEP Reports
Article Title: Differential and organ-specific functions of organic solute transporter α and β in experimental cholestasis
doi: 10.1016/j.jhepr.2022.100463
Figure Lengend Snippet: Challenging adult wild-type, Ostα -/- and Ostβ -/- mice by inducing cholestasis using a DDC diet. (A) Body weight change in Ostβ -/- and WT (right panel) and body weight change in Ostα -/- and WT littermates (left panel) after 8 days with DDC diet (n = 6-9 per group). (B) Ileal mRNA expression of Asbt , Fgf15 and hepatic mRNA expression of Cyp7a1 (n = 6-9 per group). Data are normalized using the geometric mean of CyclophillinB - Hprt (ileum) and Tbp-Hprt (liver). Adult Ostβ -/- mice were injected via the tail vein with an AAV8 vector encoding mouse OSTβ and then cholestasis was induced using a DDC diet. (C) Body weight change in Ostβ -/- WT/HET mice and Ostβ -/- (left panel) and Ostβ -/- and Ostβ -/- AAV8 (right panel) after 12 days of a DDC diet (n = 8-10 mice per group). (D) Hepatic mRNA expression of Slc51β ; data are normalized using the geometric mean of CyclophillinB-Hprt . (E) Small intestine length and (F) liver weight of adult Ostβ WT/HET mice, Ostβ -/- mice and Ostβ -/- AAV8 were measured (n = 8–10 mice per group). Statistical analysis was performed using a one-way ANOVA test, and Dunnett’s test to compare with the wild-type littermates. ∗ p values of <0.05 were considered statistically significant. AAV8, adeno-associated virus serotype 8. AAV, adeno-associated virus; Asbt , apical sodium-dependent bile acid transporter; Cyp7a1 , Cytochrome P450 Family 7 Subfamily A Member 1; DDC diet, 3.5-diethoxycarbonyl-1.4-dihydrocollidine diet; Fgf15 , fibroblast growth factor 15; HET, heterozygous; HOM, homozygous; Ostα , organic solute transporter alpha; Ostβ , organic solute transporter beta; Slc51β , solute carrier family 51, beta subunit; wk, week.
Article Snippet: In indicated experiments, DDC diet was initiated 2 weeks after administration via the tail vein of 2x10 12 adeno-associated virus serotype 8 (AAV8) particles/kg encoding codon optimized mouse OSTβ (Vectorbuilder).
Techniques: Expressing, Injection, Plasmid Preparation, Virus
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